Point-of-Care Diagnostic for Trichomonas vaginalis, the Most Prevalent, Non-Viral Sexually Transmitted Infection.

A point-of-care (POC) diagnostic is needed for both women and men to establish universal screening and surveillance for the number one, non-viral sexually transmitted infection (STI) caused by Trichomonas vaginalis. We developed a POC diagnostic for this STI using the MedMira Rapid Vertical Flow (RVF®) Technology test cartridge with a membrane that includes a Vertical procedural/reagent control line (referred to as CVL) and spotted with 1 µg of a 72.4-kDa truncated version of α-actinin called ACT::SOE3. This protein is a specific diagnostic target for antibody in sera of individuals with trichomoniasis. Serum antibody to ACT::SOE3 is a positive reaction with the test spot. Specificity of ACT::SOE3 was revealed with monoclonal antibodies (MAbs) generated to ACT::SOE3. Addition of negative control serum with MAb 67B reactive to ACT::SOE3 shows detection of both ACT::SOE3 and the CVL. Only positive sera of individuals had antibody reactive with ACT::SOE3 and detected the presence of the spot and the CVL. Negative control sera were unreactive with ACT::SOE3 and only showed the presence of the CVL. Importantly, to show proof-of-principle for POC application, ACT::SOE3 was detected with the positive patient sera spiked with whole blood. Finally, packaged cartridges stored with desiccant packs at 37 °C for one year gave identical results with the positive and negative human sera. Our results show the validity of this new POC serodiagnostic for this STI.

Microtornillos como método de control vertical en desprogramación muscular / Vertical control with miniscrews in muscular deprogramming treatment

Una de las consecuencias de la desprogramación neuromuscular es la rotación mandibular en sentido horario, evidenciando el punto prematuro de contacto y aumentando la dimensión vertical del paciente. En pacientes clase II con componente vertical, es un problema realizar este tipo de tratamiento, ya que por lo general este efecto de posterorrotación mandibular hace el perfil más convexo, y en algunos casos genera mordida abierta anterior, la cual se debe corregir posteriormente con el tratamiento de ortodoncia, cirugía o prótesis, lo que alarga el tiempo total de tratamiento por la necesidad de control vertical. El presente caso se trata de una mujer de 45 años de edad, dolicofacial, con tendencia a mordida abierta, mordida dual, sobremordida vertical y horizontal disminuidas, motivo de consulta dolor articular, el cual no le permite continuar con sus labores diarias, el tratamiento realizado fue desprogramación neuromuscular con un guarda oclusal inferior con el propósito de aliviar sintomatología articular y control vertical con microtornillos palatinos previo a tratamiento ortodóncico (AU)

One of the effects of the neuromuscular deprogramming treatment is the mandibular clockwise rotation, making the light premature occlusal contact more evident and increasing the patient vertical dimension. In Class II patients with vertical component is difficult to treat them due to profile worsening as an effect of the clockwise rotation creating in some patient's anterior open bite, this has to be corrected later in treatment with orthodontic intrusion, surgery or prosthodontic treatment, increasing the total time of treatment with the vertical control necessity. This case report is a 45 years old patient, dolichofacial, with anterior open bite tendency, dual bite, decreased overjet and overbite, her chief complaint was temporomandibular joint dysfunction which dont allow her to do her daily duties, the treatment for her was neuromuscular deprogramming splint for the temporomandibular joint pain, and vertical control with temporary anchorage devices (miniscrews) before the orthodontic treatment (AU)

Efecto del entrenamiento muscular masticatorio en el desarrollo del patrón vertical facial en niños: revisión narrativa / Training effect of the masticatory muscles in the development of the vertical facial pattern in children: narrative review

RESUMEN: Introducción: El patrón de crecimiento facial se establece antes de la aparición del primer molar permanente. Un exceso de crecimiento vertical facial, corresponde a individuos de cara larga, con ángulos goniacos abiertos, alturas faciales inferiores aumentadas y músculos masticatorios de menor área transversal. Esto se asocia a presentar mordida abierta anterior, mordida cruzada, clase II esquelético, entre otros. El entrenamiento muscular masticatorio podría controlar el excesivo crecimiento vertical, generando fuerzas opuestas que favorezcan un crecimiento horizontal. Objetivo: Describir el efecto del entrenamiento muscular masticatorio en el desarrollo del patrón vertical facial en niños. Método: Se realizó una revisión narrativa mediante búsqueda electrónica en las bases de datos PubMed, EBSCO, Scopus, Cochrane, Trip Database y Epistemonikos. Se utilizaron las palabras clave: "músculos masticatorios", "maseteros", "temporales", "masticación", "ejercicio", "entrenamiento", "crecimiento vertical", "patrón vertical", "dolicofacial", "control vertical". Los términos MeSH: "músculos masticatorios", "ejercicio". Resultados: Se seleccionaron 15 artículos de los cuales 9 son ensayos clínicos, 4 son reportes de caso y 2 son estudios observacionales transversales. Conclusiones: El entrenamiento muscular masticatorio tiene efectos positivos, favoreciendo un mayor crecimiento horizontal en niños con patrón vertical. No obstante, faltan estudios y ensayos clínicos para establecer y cuantificar los cambios morfológicos generados por el entrenamiento muscular.

ABSTRACT: Background: The pattern of facial growth is established before the eruption of the first permanent molar. An excess of vertical facial growth corresponds to individuals with long faces, open gonial angles, higher inferior facial heights and smaller masticatory muscles associated with different dental anomalies like anterior open bite, Brodie bite, skeletal class II and others. The masticatory muscle training could control the excessive growth in the vertical dimension, making opposing forces that will favor the horizontal growth in the patient. Objective: Describe the effect of the masticatory muscle training in the development of the vertical pattern in children. Method: A narrative review was done by an electronic research in PubMed, EBSCO, Scopus, Cochrane, Trip Database y Epistemonikos. The following key words were used: "masticatory muscles", "masseter", "temporalis", "mastication", "chewing", "exercise", "training", "vertical growth", "vertical pattern", "dolichofacial", "vertical control". The MeSH terms: "masticatory muscles", "exercise". Results: Fifteen articles were selected, 9 of them were clinical trials, 4 were case reports and 2 were observational studies. Conclusions: The literature review shows that the masticatory muscle training has positive effects, favoring the horizontal growth in children with vertical facial pattern. Nevertheless, there is a lack of studies and clinical trials, that could help us to establish and quantify the morphological changes made by the masticatory muscle training.

Control vertical mediante bite block en relación céntrica / Vertical control through bite block in centric relation

Paciente femenina de ocho años y cuatro meses, con patrón esquelético de clase II severa y overjet de 10 mm, fue tratada con Bite-Block Céntrico como método de control vertical. Después de cinco meses de terapia con aparatología fija de primera fase se logró control vertical y reducción de las desviaciones de la clase esquelética. Posteriormente se continuó con tratamiento activo durante 18 meses, se dio de alta de la primera fase cuando los objetivos del tratamiento como alineación, nivelación, overbite y overjet fueron adecuados. Los registros postratamiento demostraron una buena estabilidad articular, oclusión funcional y una mejora en la estética facial. El montaje en céntrica postratamiento demuestra estabilidad condilar con el uso de Bite-Block Céntrico como método de control vertical (AU)

Female patient of eight years and four months, with skeletal pattern of severe class II and 10 mm overjet, was treated with Bite-Block Centric as a vertical control method. After five months of therapy with fixed appliances of the first phase, vertical control and reduction of the deviations of the skeletal class were achieved. Subsequently continued with active treatment for 18 months, was discharged from the first phase when the treatment objectives such as alignment, leveling, overbite and overjet appropriate. Post-treatment records showed good joint stability, functional occlusion and an improvement in facial aesthetics. The posttreatment centric assembly demonstrates condylar stability with the use of Centric Bite-Block as a vertical control method (AU)